SPECTRUM CALIBRATION DEVICE AND SPECTRUM CALIBRATION METHOD

• Main Authors: KAWAGUCHI YOHEI, YASUDA TOMOHIRO, GEORGIOS CHALKIDIS
• Format: Patent
• Language: English; Japanese
• Published: 19.03.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

To provide a spectrum calibration device and a spectrum calibration method, capable of performing spectrum calibration with high accuracy even when peaks appear simultaneously between fluorescent dyes.SOLUTION: Disclosed is a spectrum calibration device for calculating a color conversion matrix 35 used for color conversion processing, which includes: a spectral signal acquisition part 31 for acquiring a spectral signal 30 of fluorescence detected over time; a candidate calculation part 33 which, based on the spectral signal, calculates a candidate for a color conversion matrix for each parameter value which depends on a frequency of simultaneous appearance of fluorescent peaks of fluorescent dyes; and a selection part 34 for selecting the color conversion matrix based on the evaluation value calculated for each candidate.SELECTED DRAWING: Figure 3 【課題】蛍光色素間でピークが同時に出現する場合であってもスペクトル校正を高精度に行えるスペクトル校正装置及びスペクトル校正方法を提供する。【解決手段】色変換処理に用いられる色変換行列３５を算出するスペクトル校正装置であって、経時的に検出された蛍光の分光信号３０を取得する分光信号取得部３１と、分光信号に基づいて、蛍光色素の蛍光のピークが同時に出現する頻度に依存するパラメータの値毎に色変換行列の候補を算出する候補算出部３３と、各候補に対して算出される評価値に基づいて色変換行列を選択する選択部３４を備えることを特徴とする。【選択図】図３