METHOD FOR EXAMINING BACTERIUM, MICRO ARRAY FOR EXAMINING BACTERIUM, KIT FOR EXAMINING BACTERIUM, PROBE SET FOR EXAMINING BACTERIUM, AND PRIMER SET FOR EXAMINING BACTERIUM

• Main Authors: ISSHIKI ATSUNORI, JUMYO SHINYA, YAMAZAKI TAKAAKI
• Format: Patent
• Language: English; Japanese
• Published: 28.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

To make it possible to detect an existing bacterium of the genus Listeria accurately and specifically in examination for checking the presence of a bacterium.SOLUTION: There is provided a method for examining the presence of a bacterium in a sample in which a target bacterium is selected from bacteria of the genus Listeria described in the following (1) to (10), (1) Listeria floridensis, (2) Listeria weihenstephanensis, (3) Listeria cornellensis, (4) Listeria riparia, (5) Listeria grandensis, (6) Listeria booriae, (7) Listeria newyorkensis, (8) Listeria aquatica, (9) Listeria fleischmannii, (10) Listeria marthii, and the method includes: (a) a preparation process of preparing reaction liquid containing a primer set for amplifying an IGS (intergenic spacer) region of rRNA gene; and (b) an amplification process of amplifying nucleic acid of the target bacterium included in a sample by using the reaction liquid obtained in the preparation process.SELECTED DRAWING: None 【課題】 細菌の存在を確認する検査において、現存するリステリア属菌を精度高く特異的に検出することを可能とする。【解決手段】 試料中における細菌の存在を検査する方法であって、対象となる細菌は、以下の（１）〜（１０）に記載のリステリア属菌から選ばれ、（１）Listeria floridensis、（２）Listeria weihenstephanensis、（３）Listeria cornellensis、（４）Listeria riparia、（５）Listeria grandensis、（６）Listeria booriae、（７）Listeria newyorkensis、（８）Listeria aquatica、（９）Listeria fleischmannii、（１０）Listeria marthii、前記方法は、（ａ）ｒＲＮＡ遺伝子のＩＧＳ（intergenic spacer）領域の増幅用プライマーセットを含む反応液を調製する調製工程、及び、（ｂ）調製工程により得られた反応液を使用して、試料に含まれる対象となる細菌の核酸を増幅する増幅工程を含む細菌の検査方法とする。【選択図】 なし