CAPILLARY-ELECTROPHORESIS-MASS SPECTROMETRY METHOD

• Main Authors: ABE KAORI, SASAKI KAZUNORI
• Format: Patent
• Language: English; Japanese
• Published: 21.11.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

To stabilize migration time MT while omitting a cleaning process by a cleaning buffer to reduce measurement time.SOLUTION: When measuring a sample 8 by a capillary-electrophoresis (CE)-mass spectrometry (MS) method using a migration liquid 16, variation of migration time MT is relaxed by including a material interacting with metal ions, a material (a metal chelate agent, for example, acetyl acetone) capable of maintaining a state of dissolving the metal ions included in the sample 8 or a material (for example, phosphoric acid) capable of reducing the solubility of the metal ions included in the sample 8, in the migration liquid 16.SELECTED DRAWING: Figure 5 【課題】洗浄バッファによる洗浄過程を省略しつつ泳動時間ＭＴを安定化し、測定時間を短縮する。【解決手段】泳動液１６を用いてキャピラリー電気泳動（ＣＥ）−質量分析（ＭＳ）方法により試料８を測定する際に、金属イオンと相互作用する物質、試料８中に含まれる金属イオンを溶存状態に保つことが可能な物質（金属キレート剤、例えばアセチルアセトン）、又は、試料８中に含まれる金属イオンの溶解度を下げる物質（例えばリン酸）を前記泳動液１６に含有させることによって泳動時間ＭＴの変動を緩和する。【選択図】図５