HYDROGENOPHILACEAE BACTERIA TRANSFORMANT

• Main Authors: YUGAWA HIDEAKI, ISHII MASAHARU, OTANI NAOTO
• Format: Patent
• Language: English; Japanese
• Published: 07.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide a transformant that makes it possible to produce efficiently ethanol with carbon dioxide used as a single carbon source.SOLUTION: The transformant is obtained by introducing, to Hydrogenophilaceae bacteria, an alcohol dehydrogenase gene, and DNA selected from the followings: DNA comprising a specific base sequence; DNA comprising a base sequence having at least 90% identity with the DNA and encoding a polypeptide that has a pyruvate decarboxylase (PDC) activity; DNA hybridizing with a base sequence complimentary to the specific base sequence under a stringent condition, and encoding a polypeptide that has a PDC activity; DNA encoding a polypeptide that has a specific amino acid sequence; DNA having at least 90% identity with the specific amino acid sequence and encoding a polypeptide that has a PDC activity; or DNA comprising a specific amino acid sequence in which one or plural amino acids are deleted, substituted or added and encoding a polypeptide that has a PDC activity.SELECTED DRAWING: None 【課題】二酸化炭素を唯一の炭素源として利用して効率よくエタノールを製造することができる形質転換体などの提供。【解決手段】ヒドロゲノフィラス属細菌に、特定の塩基配列からなるＤＮＡ、該ＤＮＡと９０％以上の同一性を有する塩基配列でかつピルビン酸デカルボキシラーゼ（ＰＤＣ）活性を有するポリペプチドをコードするＤＮＡ、特定の塩基配列と相補的な塩基配列とストリンジェントな条件でハイブリダイズしかつＰＤＣ活性を有するポリペプチドをコードするＤＮＡ、特定のアミノ酸配列からなるポリペプチドをコードするＤＮＡ、特定のアミノ酸配列と９０％以上の同一性を有しかつＰＤＣ活性を有するポリペプチドをコードするＤＮＡ、あるいは、１個又は複数個のアミノ酸が欠失、置換、又は付加された特定のアミノ酸配列でＰＤＣ活性を有するポリペプチドをコードするＤＮＡ、及びアルコールデヒドロゲナーゼ遺伝子を導入した形質転換体。【選択図】なし