RNA INTERFERENCE AGENTS FOR GST-π GENE MODULATION

• Main Authors: MOHAMMAD AHMADIAN, JENS HARBORTH, TAKAHASHI HIROICHI, ZHANG JUN, AJINOMI KENJIRO, TERADA ERIKA, YING WENBIN
• Format: Patent
• Language: English; Japanese
• Published: 31.10.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

To provide compounds for modulating human GST-π using RNA interference.SOLUTION: Disclosed herein is a nucleic acid molecule that inhibits expression of GST-π comprising a sense strand and an antisense strand and forms a double stranded region, where the antisense strand comprises a nucleotide sequence of a specific sequence with or without modification, the sense strand comprises a nucleotide sequence of a specific sequence with or without modification (in these nucleotide sequences, A, G, C and U are ribo-A, ribo-G, ribo-C and ribo-U respectively, N is A, C, G, U, 2'-OMe-substituted U, a, c, g, u, t, modified nucleotide, inverted nucleotide or chemically modified nucleotide where a, c, g, u and t are 2'-deoxy-A, 2'-deoxy-C, 2'-deoxy-G, 2'-deoxy-U and 2'-deoxy-T respectively.SELECTED DRAWING: Figure 1 【課題】ＲＮＡ干渉を利用してヒトＧＳＴ−πの発現を調節する化合物の提供。【解決手段】センス鎖及びアンチセンス鎖を含むＧＳＴ−πの発現を阻害する核酸分子であって、前記鎖が二重鎖領域を形成し、前記アンチセンス鎖は特定の配列からなるヌクレオチド配列又は修飾を有する当該ヌクレオチド鎖を含み、前記センス鎖は特定の配列からなるヌクレオチド配列又は修飾を有する当該ヌクレオチド鎖を含む（これらヌクレオチド配列において、Ａ、Ｇ、Ｃ及びＵはそれぞれリボＡ、リボＧ、リボＣ及びリボＵであり、ＮはＡ、Ｃ、Ｇ、Ｕ、２'−ＯＭｅ置換Ｕ、ａ、ｃ、ｇ、ｕ、ｔ、修飾ヌクレオチド、逆方向（ｉｎｖｅｒｔｅｄ）ヌクレオチド又は化学修飾ヌクレオチドである（ａ、ｃ、ｇ、ｕ及びｔはそれぞれ２'−デオキシ−Ａ、２'−デオキシ−Ｃ、２'−デオキシ−Ｇ、２'−デオキシ−Ｕ及び２'−デオキシ−Ｔ））、核酸分子。【選択図】図１