NUCLEIC ACID SAMPLE CONTAINING VESSEL, PRODUCTION METHOD OF NUCLEIC ACID SAMPLE CONTAINING VESSEL, PRODUCTION DEVICE OF NUCLEIC ACID SAMPLE CONTAINING VESSEL AND NUCLEIC ACID SAMPLE CONTAINING PROGRAM, AND NUCLEIC ACID SAMPLE

• Main Authors: SEO MANABU, KAWASHIMA YUDAI, YUMOTO MASAYUKI, IZUMI KEN
• Format: Patent
• Language: English; Japanese
• Published: 26.09.2019
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide a nucleic acid sample containing vessel in which a desired molecule number (copy number) of nucleic acids including a prescribed number of target base sequences are dispensed into a vessel.SOLUTION: There is provided a nucleic acid sample containing vessel 1 comprising: a prescribed number of first nucleic acid molecules comprising target base sequences and detection base sequences different from the target base sequences; and second nucleic acid molecules having no target base sequence and having the detection base sequences. A copy number of the target base sequences is less than 1000 and a variation coefficient (CV value) of the copy number is less than 20%, preferably, the nucleic acid molecule is preferably an artificial synthetic nucleic acid molecule, and the first nucleic acid molecule preferably comprises a plurality of target base sequences in a same molecule.SELECTED DRAWING: Figure 2 【課題】所定数の目的の塩基配列を含む核酸を、所望の分子数（コピー数）だけ容器に分注された核酸試料含有容器を提供する。【解決手段】目的の塩基配列及び目的の塩基配列とは異なる検出用塩基配列を有する第一の核酸分子の所定数と、目的の塩基配列を有さず、検出用塩基配列を有する第二の核酸分子と、を含有する核酸試料含有容器１である。目的の塩基配列のコピー数が１，０００未満かつ、コピー数の変動係数（ＣＶ値）が２０％未満である態様が好ましく、前記核酸分子が人工合成核酸分子である態様がより好ましく、第一の核酸分子が、目的の塩基配列を同一分子内に複数有する態様が更に好ましい。【選択図】図２