CD19-SPECIFIC CHIMERIC ANTIGEN RECEPTOR AND USES THEREOF

• Main Authors: ANDREW SCHARENBERG, GALETTO ROMAN, CECILE SCHIFFER-MANNIOUI, JULIANNE SMITH
• Format: Patent
• Language: English; Japanese
• Published: 25.07.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

To provide: a chimeric antigen receptor (CAR) which comprises an extracellular ligand binding domain, a transmembrane domain and a signaling domain; and methods for engineering immune cells into which the receptor is introduced.SOLUTION: A CD19-specific chimeric antigen receptor comprises at least one extracellular ligand binding domain, a transmembrane domain, and at least one intracellular signaling domain, where the extracellular domain comprises a single chain FV fragment derived from a γ heavy chain and a κ light chain of a CD19-specific monoclonal antibody 4G7.SELECTED DRAWING: Figure 1 【課題】細胞外リガンド結合ドメイン、膜貫通ドメイン、およびシグナリング伝達ドメインを含むキメラ抗原受容体（CAR）の提供、及び該受容体が導入された免疫細胞を操作する方法。【解決手段】少なくとも1つの細胞外リガンド結合ドメイン、膜貫通ドメイン、および少なくとも1つの細胞内シグナリングドメインを含むCD19特異的キメラ抗原受容体であって、該細胞外ドメインが、CD19に特異的なモノクローナル抗体4G7のγ重鎖およびκ軽鎖に由来する単鎖FV断片を含む、CD19特異的キメラ抗原受容体。【選択図】図１