METHODS AND COMPOSITIONS FOR ENHANCING NUCLEASE-MEDIATED GENE DISRUPTION

• Main Authors: KANG QIAOHUA, CHEN FUQIANG, THOMAS WECHSLER
• Format: Patent
• Language: English; Japanese
• Published: 11.07.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

To provide methods and compositions for increasing nuclease-mediated genomic modification using DNA repair inhibitors.SOLUTION: In one aspect, a method for targeted genomic disruption in a cell is provided, the method comprising: administering at least one nuclease to the cell, where the nuclease cleaves endogenous genomic sequences in the cell; administering to the cell at least one inhibitor of classic or alternative non-homologous end joining (NHEJ).SELECTED DRAWING: None 【課題】ＤＮＡ修復阻害剤を使用してヌクレアーゼ媒介性ゲノム修飾を増加させるための方法および組成物を提供すること。【解決手段】一局面において、細胞における標的化されたゲノム破壊のための方法が提供され、上記方法は：少なくとも１種のヌクレアーゼを上記細胞に投与するステップであって、上記ヌクレアーゼが、上記細胞における内因性ゲノム配列を開裂する、ステップと、典型的または代替的非相同末端結合（ＮＨＥＪ）の少なくとも１種の阻害剤を上記細胞に投与するステップとを含む。【選択図】なし