ONE BASE POLYMORPHISM DETECTION PROBE DESIGN METHOD AND PROBE SET

• Main Authors: TSUDA TOSHIYA, OBA MITSUYOSHI, YAMANO HIROBUMI
• Format: Patent
• Language: English; Japanese
• Published: 04.07.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

To suppress generation of cross hybridization and accurately detect a target of perfect matching.SOLUTION: The invention is configured to calculate a coupling ratio with a wild target or variant target of a candidate wild probe, and a coupling ratio with a variant target or wild target of a candidate variant probe, then design so that, a value obtained by subtracting the coupling ratio with the candidate variant probe from the coupling ratio with the candidate wild probe, about the wild target, and a value obtained by subtracting the coupling ratio with the candidate wild probe from the coupling ratio with the candidate variant probe about the variant target, become a prescribed value or greater value, respectively.SELECTED DRAWING: None 【課題】クロスハイブリダイゼーションの発生を抑え、完全一致のターゲットを高精度に検出する。【解決手段】候補野生型プローブについて野生型ターゲット又は変異型ターゲットとの結合率、候補変異型プローブについて変異型ターゲット又は野生型ターゲットとの結合率を計算し、野生型ターゲットに関して、候補野生型プローブとの結合率から候補変異型プローブとの結合率を引いた値、変異型ターゲットに関して、候補変異型プローブとの結合率から候補野生型プローブとの結合率を引いた値を所定の値以上となるように設計する。【選択図】なし