FC-BINDING PROTEINS WITH AMINO ACID SUBSTITUTION

• Main Authors: OTAKE RYOKO, OE MASATAKE, TERAO YOSUKE
• Format: Patent
• Language: English; Japanese
• Published: 23.05.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide FcγRIIIa with amino acid substitution of which productivity in a recombinant is improved as compared with conventional FcγRIIIa.SOLUTION: Disclosed herein is an FcγRIIIa having the amino acid sequence of human FcγRIIIa (GenBank AAH17865.1) with amino acid substitution at least at position 176 to any of isoleucine, alanine and tyrosine.SELECTED DRAWING: None 【課題】 従来のＦｃγＲＩＩＩａと比較し、遺伝子組換体による生産性が向上した、ＦｃγＲＩＩＩａアミノ酸置換体を提供すること。【解決手段】 ヒトＦｃγＲＩＩＩａ（ＧｅｎＢａｎｋ ＡＡＨ１７８６５．１）のアミノ酸配列のうち、少なくとも１７６番目のアミノ酸残基をイソロイシン、アラニン、チロシンのいずれかに置換することで、前記課題を解決する。【選択図】 なし