CELL CULTURE TANK

• Main Authors: ISHII KOSUKE, TSUJIKAWA JUN
• Format: Patent
• Language: English; Japanese
• Published: 16.05.2019
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

To inhibit the fusion of a cell cluster having a desired size.SOLUTION: A culture tank 20 comprises: an upper layer portion 24 for growing cell mass; a lower layer portion 22 provided lower than the upper layer portion 24 and receiving a culture medium M and flowing the culture medium M toward the upper layer portion 24; and a middle layer portion 23 provided between the upper layer portion 24 and the lower layer portion 22 and flowing the culture medium M received from the lower layer portion 22 upward toward the upper layer portion 24. The rising velocity component of the flow of the culture medium M in the lower layer portion 22 is decelerated from a first velocity (V1) to a second velocity (V2) slower than the first velocity (V1). The rising velocity component of the flow of the culture medium M in the middle layer portion 23 maintains the second velocity (V2). The rising velocity component of the flow of the culture medium M in the upper layer portion 24 is decelerated from the second velocity (V2) to a third velocity (V3) slower than the second velocity (V2).SELECTED DRAWING: Figure 1 【課題】所望の大きさを有する細胞塊の融合を抑制する。【解決手段】培養槽２０は、細胞塊を成長させる上層部２４と、上層部２４よりも下方に設けられ、培地Ｍを受け入れて培地Ｍを上層部２４に向けて流す下層部２２と、上層部２４と下層部２２との間に設けられ、下層部２２から受け入れた培地Ｍを上層部２４に向けて上方に流す中層部２３と、を備える。下層部２２において培地Ｍの流れが有する上昇速度成分は、第１速度（Ｖ１）から第１速度（Ｖ１）よりも遅い第２速度（Ｖ２）に減速する。中層部２３において培地Ｍの流れが有する上昇速度成分は、第２速度（Ｖ２）を維持する。上層部２４において培地Ｍの流れが有する上昇速度成分は、第２速度（Ｖ２）から第２速度（Ｖ２）よりも遅い第３速度（Ｖ３）に減速する。【選択図】図１