RECOMBINANT CLOSTRIDIUM BOTULINUM NEUROTOXINS

• Main Authors: MATTHEW BEARD, AIMEE COSSINS, MARKS PHILIP
• Format: Patent
• Language: English; Japanese
• Published: 09.05.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide improved methods for producing recombinant Clostridium botulinum (C. botulinum) neurotoxins (BoNT/E), in particular, active di-chain BoNT recombinant BoNT/E.SOLUTION: Disclosed herein is a nucleic acid sequence comprising a sequence of contiguous nucleotides, where the sequence of contiguous nucleotides has at least 90% sequence identity to a nucleic acid sequence optimized for expression and encodes a single-chain BoNT/E1 protein. Also disclosed are methods for producing a soluble single-chain BoNT/E1 protein in an E. coli host cell, together with methods for producing a soluble di-chain BoNT/E1 protein.SELECTED DRAWING: None 【課題】組換えクロストリジウムボツリヌス(C.botulinum)神経毒（BoNT/E）、特に活性2本鎖BoNT組換えBoNT/Eを製造するための改良された方法を提供する。【解決手段】発現に最適化された核酸配列に対して少なくとも90％の配列同一性を有し且つ単鎖BoNT/E1タンパク質をコードする近接ヌクレオチドの配列を含む核酸配列を開示する。更に、E.coli宿主細胞において可溶性単鎖BoNT/E1タンパク質を製造する方法を、可溶性2本鎖BoNT/E1タンパク質を製造する方法と併せて開示する。【選択図】なし