AGGLUTINATION IMMUNOASSAY

• Main Authors: YOSHIDA TADAAKI, TAKAHASHI HIROYUKI, TAKAHASHI YUKI, BAMBA YOSHIMASA
• Format: Patent
• Language: English; Japanese
• Published: 18.04.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

To provide a particle enhanced agglutination immunoassay capable of high sensitivity and wide dynamic range measurement.SOLUTION: A particle enhanced agglutination immunoassay includes the steps of: mixing sample solution containing an analyte with solution containing insoluble carrier particles carrying a binding partner for the analyte to prepare mixed solution; measuring a variation amount (i) in intensity of scattered light scattered from the mixed solution from difference in intensity of scattered light between first and second time points; measuring a variation amount (ii) in absorbance of the mixed solution from difference in absorbance between third and fourth time points; and correlating the measured variation amount (i) in intensity of scattered light and the measured variation amount (ii) in absorbance with an amount of the analyte present in the sample by using a calibration curve based on the variation amount in intensity of scattered light and a calibration curve based on the variation amount in absorbance. The present invention employs measurements of the intensity of scattered light and measurements of the absorbance in combination for a single assay, and thus can provide a particle enhanced agglutination immunoassay which achieves higher sensitivity and a wider dynamic range than conventional assays.SELECTED DRAWING: Figure 3 【課題】高感度かつダイナミックレンジの広い測定の可能な粒子増強免疫凝集測定法。【解決手段】アナライトを含む試料溶液と、アナライトとの結合パートナーを担持した不溶性担体粒子を含む溶液とを混合して混合液を調製する工程と；第１、第２の時点間の散乱光強度差から前記混合液の（イ）散乱光強度の変化量を測定する工程と；第３、第４の時点間の吸光度差から前記混合液の（ロ）吸光度の変化量を測定する工程と；測定された前記（イ）散乱光強度の変化量および前記（ロ）吸光度の変化量を、散乱光強度変化量に基づく検量線および吸光度変化量に基づく検量線を用いて試料中のアナライトの存在量と関連付ける工程と、を具備する粒子増強免疫凝集測定法。散乱光強度測定と吸光度測定を一つの測定において併用することにより、従来よりも高感度かつダイナミックレンジの広い粒子増強免疫凝集測定法の提供を可能とした。【選択図】図３