APPARATUS FOR CULTURING NEURONS, METHOD FOR CULTURING NEURONS, CULTURED NEURONS, METHOD FOR ANALYZING AND IDENTIFYING PROTEIN IN AXON BUNDLE, AND METHOD FOR USING NEURONS

To provide apparatus and methods for culturing neurons allowing an axon bundle extended from a neuron to grow rapidly in vitro.SOLUTION: Provided is an apparatus for culturing neuronal assembly having a spheroid and an axon bundle extending in one direction from the spheroid, comprising a culture pl...

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Bibliographic Details
Main Authors KANEDA SHOHEI, KAWADA HARUYOSHI, IKEUCHI YOSHIHO, FUJII TERUO
Format Patent
LanguageEnglish
Japanese
Published 14.03.2019
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:To provide apparatus and methods for culturing neurons allowing an axon bundle extended from a neuron to grow rapidly in vitro.SOLUTION: Provided is an apparatus for culturing neuronal assembly having a spheroid and an axon bundle extending in one direction from the spheroid, comprising a culture plate, and a plurality of modules arranged in the culture plate, each module comprising a first chamber capable of receiving a cell body of a neuron, a second chamber, and a channel which is capable of receiving the axis bundle extending from the cell body and contacts the first chamber with the second chamber, the lower end of the first chamber, the second chamber, and the channel being closed, the upper end of the first chamber and the second chamber being opened, the channel having a length of 1 [mm] or more, and the neuronal assembly being cultured in the state where it can be extracted one by one in each module.SELECTED DRAWING: Figure 1 【課題】in vitroで神経細胞から延びる軸索束を迅速に成長させる、神経細胞の培養装置、培養方法の提供。【解決手段】１つのスフェロイドと、スフェロイドから１方向に延びる１つの軸索束とを有する神経細胞の密集体を培養する装置であって、培養プレートと、培養プレート内に配置された複数のモジュールであって、各モジュールは、神経細胞の細胞体を受容可能な第１チャンバーを１つ、第２チャンバーを１つ、及び、細胞体から延びた軸索束を受容可能なチャネルであって第１チャンバーと第２チャンバーとを接続するチャネルを１つ含むモジュールと、を備え、第１チャンバー、第２チャンバー及びチャネルの下端が閉止され、第１チャンバー及び第２チャンバーの上端が開放され、チャネルは、長さ１〔ｍｍ〕以上であって、神経細胞の密集体は、各モジュール内で１つずつ抽出することができる状態で培養される。【選択図】図１
Bibliography:Application Number: JP20180205589