CELL SEPARATION DEVICE AND METHOD OF OBTAINING CELL-CONTAINING LIQUID COMPRISING MONOCYTES

• Main Author: YAMASHITA KEITA
• Format: Patent
• Language: English; Japanese
• Published: 21.02.2019
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide a cell separation device for recovering monocytes with good recovery efficiency while removing granulocytes and erythrocytes and a method of obtaining a cell-containing liquid comprising monocytes using said cell separation device.SOLUTION: When obtaining a cell-containing liquid comprising monocytes while removing granulocytes and erythrocytes from a crude cell-containing liquid comprising monocytes, granulocytes and erythrocytes, a cell separation device is used in which a first filter and a second filter are provided in series where filter members having a prescribed permeability and comprising unwoven fibers are respectively loaded inside the first filter and the second filter in respectively prescribed states.SELECTED DRAWING: Figure 3 【課題】顆粒球、及び赤血球を除去しつつ、良好な回収効率で単核球を回収できる細胞分離装置と、当該細胞分離装置を用いて単核球を含む細胞含有液を取得する方法とを提供すること。【解決手段】単核球、顆粒球、及び赤血球を含む粗細胞含有液から、顆粒球、及び赤血球を除去しつつ、単核球を含む細胞含有液を取得する際に、直列につながれた第１フィルターと第２フィルターとを備え、第１フィルター内、及び第２フィルター内に、それぞれ所定の通気度を示す不織布からなるフィルター部材が、それぞれ所定の状態で充填された細胞分離装置を用いる。【選択図】図３