METHOD FOR PRODUCING GLYCOPROTEIN

To provide a technique that improves productivity of glycoprotein.SOLUTION: A fusion protein of a structure in which a peptide, found as a common sequence of a blood coagulation factor V and a blood coagulation factor VIII, is linked to a carboxy terminal side of a desired glycoprotein is expressed...

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Bibliographic Details
Main Authors YAGI HIROKAZU, HONDA RENA, KATO KOICHI
Format Patent
LanguageEnglish
Japanese
Published 31.01.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:To provide a technique that improves productivity of glycoprotein.SOLUTION: A fusion protein of a structure in which a peptide, found as a common sequence of a blood coagulation factor V and a blood coagulation factor VIII, is linked to a carboxy terminal side of a desired glycoprotein is expressed in a cultured mammalian cell, and a fusion protein is recovered from a culture medium.SELECTED DRAWING: None 【課題】本発明は、糖タンパク質の生産性を向上させる技術を提供することを課題とする。【解決手段】血液凝固第5因子と第8因子に共通する配列として見出されたペプチドが目的糖タンパク質のカルボキシ末端側に連結が連結された構造の融合タンパク質を培養哺乳動物細胞内で発現させ、培養液から融合タンパク質を回収する。【選択図】なし
Bibliography:Application Number: JP20170132312