CELL CULTURE TANK AND CELL CULTURE APPARATUS

• Main Authors: SAKAI SHINICHI, ISHII KOSUKE
• Format: Patent
• Language: English; Japanese
• Published: 10.01.2019
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

To suppress reduction of cell quality while controlling the size of a cell cluster.SOLUTION: A cell culture tank 2 comprises: a tank body 8 which houses culture medium M; a pump 12 which is connected to the tank body 8 and generates a flow of the culture medium M; a blade unit 30 which divides a cell cluster C on a bottom part of the tank body 8; a controller 40 which controls the pump 12. The controller 40 activates the pump 12 so as to make a flow state of the culture medium M become a culture state. Then, the controller 40 switches the action of the pump 12 such that the flow state of the culture medium M becomes a divided state different from the culture state. The divided state has a smaller rising speed of the culture medium M with respect to the culture state.SELECTED DRAWING: Figure 1 【課題】細胞品質の低下を抑制しつつ細胞塊の大きさを制御する。【解決手段】細胞培養槽２は、培地Ｍを収容する槽本体８と、槽本体８に接続され、培地Ｍの流れを生じさせるポンプ１２と、槽本体８の底部において、細胞塊Ｃを分断するブレードユニット３０と、ポンプ１２を制御する制御装置４０と、を備える。制御装置４０は、培地Ｍの流れ状態を培養状態とするようにポンプ１２を動作させる。その後、制御装置４０は、培地Ｍの流れ状態を培養状態とは異なる分断状態となるようにポンプ１２の動作を切り替える。分断状態は、培養状態に対して培地Ｍの上昇速度が小さい。【選択図】図１