ONCOLYTIC ADENOVIRUS CODING B7 PROTEIN
PROBLEM TO BE SOLVED: To provide an oncolytic adenovirus for treating cancer containing transgene which codes B7 protein being a type of superficial membrane protein that exists in an activated antigen-presenting cell (APC).SOLUTION: A replicable oncolytic group B adenovirus EnAd contains, under the...
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Main Authors | , |
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Format | Patent |
Language | English Japanese |
Published |
13.09.2018
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Subjects | |
Online Access | Get full text |
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Summary: | PROBLEM TO BE SOLVED: To provide an oncolytic adenovirus for treating cancer containing transgene which codes B7 protein being a type of superficial membrane protein that exists in an activated antigen-presenting cell (APC).SOLUTION: A replicable oncolytic group B adenovirus EnAd contains, under the control of a main late promoter which is inherent in the virus, 2 or more transgene, first transgene containing a DNA sequence which codes B7-1 protein, and additional transgene which codes anti CD3 antibody or a binding fragment thereof, in which the transgene is in the same position between a stop codon and a poly-A recognition site of adenoviral gene L5 and a stop codon and a poly-A recognition site of gene E4.SELECTED DRAWING: Figure 6
【課題】癌治療のための、活性化抗原提示細胞(APC)にみられる表在性膜タンパク質の一種であるB7タンパク質をコードする導入遺伝子を含む腫瘍溶解性アデノウイルスの提供。【解決手段】複製可能な腫瘍溶解性B群アデノウイルスEnAdであって、前記ウイルスに内在する主要後期プロモーターの制御下に、2以上の導入遺伝子、B7−1タンパク質をコードするDNA配列を含む第一の導入遺伝子、及び抗CD3抗体又はその結合フラグメントをコードする追加の導入遺伝子を含み、前記導入遺伝子がアデノウイルス遺伝子L5の停止コドン及びポリA認識部位と遺伝子E4の停止コドン及びポリA認識部位との間の同じ位置にあるウイルス。【選択図】図6 |
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Bibliography: | Application Number: JP20180041788 |