ONCOLYTIC ADENOVIRUS CODING B7 PROTEIN

• Main Authors: BRIAN ROBERT CHAMPION, ALICE CLAIRE NOEL BROMLEY
• Format: Patent
• Language: English; Japanese
• Published: 13.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide an oncolytic adenovirus for treating cancer containing transgene which codes B7 protein being a type of superficial membrane protein that exists in an activated antigen-presenting cell (APC).SOLUTION: A replicable oncolytic group B adenovirus EnAd contains, under the control of a main late promoter which is inherent in the virus, 2 or more transgene, first transgene containing a DNA sequence which codes B7-1 protein, and additional transgene which codes anti CD3 antibody or a binding fragment thereof, in which the transgene is in the same position between a stop codon and a poly-A recognition site of adenoviral gene L5 and a stop codon and a poly-A recognition site of gene E4.SELECTED DRAWING: Figure 6 【課題】癌治療のための、活性化抗原提示細胞（ＡＰＣ）にみられる表在性膜タンパク質の一種であるＢ７タンパク質をコードする導入遺伝子を含む腫瘍溶解性アデノウイルスの提供。【解決手段】複製可能な腫瘍溶解性Ｂ群アデノウイルスＥｎＡｄであって、前記ウイルスに内在する主要後期プロモーターの制御下に、２以上の導入遺伝子、Ｂ７−１タンパク質をコードするＤＮＡ配列を含む第一の導入遺伝子、及び抗ＣＤ３抗体又はその結合フラグメントをコードする追加の導入遺伝子を含み、前記導入遺伝子がアデノウイルス遺伝子Ｌ５の停止コドン及びポリＡ認識部位と遺伝子Ｅ４の停止コドン及びポリＡ認識部位との間の同じ位置にあるウイルス。【選択図】図６