CELL DENSITY MEASUREMENT METHOD AND CELL DENSITY CHANGE TRACKING METHOD

• Main Author: TSUJI HAJIME
• Format: Patent
• Language: English; Japanese
• Published: 16.08.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a cell density measurement method of a cell in a cell culture, which is sensitive to only a living cell and is not affected by a dead cell, which provides the cell density and the number of cells by converting a dielectric constant and thus not requiring sampling, and allowing measurement and evaluation to be directly incorporated into the process.SOLUTION: The cell density measurement method of a cell in a cell culture is provided in which two electrodes made from metal plate and arranged opposite to each other at a fixed distance are immersed in a culture medium, and an AC signal is applied to the electrodes to measure cell density. The method includes: signal application means applying, to the cell, an AC signal in which N number of cycles Tis used as the measurement cycle, N being between a cycle from Tto T(T>T), sequentially in the order of a long cycle; measurement means measuring the dielectric constant εwith respect to the applied cycle Tdue to the orientation polarization generated within the cells by implementing the signal application means; and calculation means for obtaining the cell number ratio for each cycle from the application cycle Tand the dielectric constant εobtained by the measurement means.SELECTED DRAWING: None 【課題】 本発明は死んでいる細胞には影響を受けずに、生きている生細胞のみに感度があり、その密度、細胞数を誘電率から換算することで求めるもので、サンプリングの必要がなく、プロセスに直接組み入れて測定、評価できる細胞培養中の細胞の細胞密度測定方法を提供する。【解決手段】 一定の距離を有して対向配置した２枚の金属板から成る電極を、細胞を培養する培地内に浸漬し、前記電極に交流信号を印可して、細胞培養中の細胞の細胞密度測定方法であって、前記細胞に周期ＴＨからＴＬ（ＴＨ＞ＴＬ）間のＮ個の周期ＴＪを測定周期とする交流信号を、長い周期の交流信号から順次与える信号印可手段、その信号印可手段の実施により細胞内で生じる配向分極による印可周期ＴＪに対する誘電率εＪを測定する計測手段、その計測手段で得られた印可周期ＴＪと誘電率εＪから、周期毎の細胞個数比率を求める計算手段を含むことを特徴とする細胞密度測定方法。【選択図】 なし