METHOD FOR PRODUCING TRIPLOBLASTIC ORGANISM

• Main Authors: SHINOHARA MARIE, SHIMIZU KOHEI, SAKAI YASUYUKI, IBUKI MASATO
• Format: Patent
• Language: English; Japanese
• Published: 02.08.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for producing any triploblastic organism, which includes culturing pluripotent stem cells, allowing efficient differentiation induction while reducing an amount of use of differentiation inducers.SOLUTION: A method for producing any triploblastic organism uses a culture device that has a first region including a culture medium and a differentiation inducer and a second region including at least part of the culture medium, with the first region and the second region separated from each other with a film, to culture pluripotent stem cells in the first region.SELECTED DRAWING: None 【課題】多能性幹細胞を培養することを含む三胚葉のいずれかの製造方法であって、分化誘導因子の使用量を低減しつつ効率的な分化誘導を可能とする方法を提供すること。【解決手段】培地と分化誘導因子とを含む第一領域と、培地成分の少なくとも一部を含む第二領域とを有し、前記第一領域と前記第二領域とが膜で隔離されている培養装置を用いて前記第一領域において多能性幹細胞を培養することを含む、三胚葉のいずれかの製造方法。【選択図】なし