CELL FORMULATION PRODUCTION METHOD AND APPARATUS AS WELL AS CELL FORMULATION PRODUCED BY PRODUCTION APPARATUS

• Main Authors: MATSUMURA JUNICHI, TSUDA YUICHIRO, HARIMOTO KENICHI, SAKAGUCHI HIROKAZU
• Format: Patent
• Language: English; Japanese
• Published: 10.05.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide cell formulation production methods and apparatus which efficiently concentrate a liquid comprising cells, and cell formulations produced by the production apparatus.SOLUTION: A cell formulation production apparatus 1 comprises a concentration unit 5 which filters cells by passing a liquid comprising cells through the interior of a hollow fiber membrane and increases the number of the cells comprised in a unit volume, a supply pump 4 which supplies the liquid comprising cells and a washing liquid into the interior of the hollow fiber membrane, and a cell sap bag 2 which stores the liquid comprising cells, being configured so that the liquid circulates the supply pump 4, the concentration unit 5, and the cell sap bag 2 in this order and the liquid level of the cell sap bag 2 is higher or more than the top end portion of the hollow fiber membrane.SELECTED DRAWING: Figure 1 【課題】細胞が含まれる液体を効率的に濃縮する細胞製剤の製造方法および製造装置、およびこの製造装置で製造された細胞製剤を提供することである。【解決手段】細胞製剤製造装置１は、細胞が含まれる液体を中空糸膜の内部を通過させることで細胞をろ過して単位体積中に含まれる細胞数を増加させる濃縮ユニット５と、細胞が含まれる液体および洗浄液を中空糸膜の内部に供給する供給ポンプ４と、細胞が含まれる液体を貯留する細胞液バッグ２と、を備え、供給ポンプ４、濃縮ユニット５、細胞液バッグ２の順に循環し、細胞液バッグ２の液面を、中空糸膜の上端部以上の高さにするものである。【選択図】図１