METHOD OF PREPARING MONOCYTES

PROBLEM TO BE SOLVED: To remove platelets from apheresis blood.SOLUTION: Monocytes are prepared so as not to include platelets. The present invention provides a method of preparing monocytes including a step of: (1) preparing apheresis blood to which is blended a non-cytotoxic, non-ionic density reg...

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Bibliographic Details
Main Authors HARADA YUI, YONEMITSU YOSHIKAZU
Format Patent
LanguageEnglish
Japanese
Published 15.03.2018
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To remove platelets from apheresis blood.SOLUTION: Monocytes are prepared so as not to include platelets. The present invention provides a method of preparing monocytes including a step of: (1) preparing apheresis blood to which is blended a non-cytotoxic, non-ionic density regulator such that the density of the blood plasma is 1.066 g/ml to 1.078 g/ml; and (2) separating monocytes by centrifuging a layered product comprising the following layer a and layer b. Layer a: density gradient centrifugation medium for separating monocytes. Layer b: density adjusted apheresis blood below layer a.SELECTED DRAWING: None 【課題】アフェレーシス血液から血小板を除去する。【解決手段】血小板を含まないように単核球を調製する。本発明は、（１）アフェレーシス血液に、細胞非障害性であり、かつ非イオン性である密度調節剤を混合し、血漿の密度を１．０６６ｇ／ｍｌ以上１．０７８ｇ／ｍｌ以下 に調整し；そして（２）下記ａ層およびｂ層を含む重層物を遠心して、単核球を分離する工程を含む、単核球の調製方法を提供する：ａ．単核球分離用密度勾配遠心媒体の層、ｂ．ａ層の下の、密度調整したアフェレーシス血液の層。【選択図】なし
Bibliography:Application Number: JP20160176379