NUCLEIC ACID AMPLIFICATION DEVICE, NUCLEIC ACID AMPLIFICATION METHOD, AND CHIP FOR NUCLEIC ACID AMPLIFICATION

• Main Authors: NAGAI SHUSUKE, HAGIWARA YOSHIHISA, FUCHIWAKI YUSUKE, FURUYA SHUNSUKE
• Format: Patent
• Language: English; Japanese
• Published: 08.03.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a portable compact reciprocal flow type nucleic acid amplification device capable of high speed real-time PCR.SOLUTION: The nucleic acid amplification device is provided with: a heater capable of forming a denaturing temperature zone and an elongation/annealing temperature zone; a fluorescence detector capable of detecting the transfer of a sample solution between the two temperature zones; a pair of fluid supply mechanisms capable of transferring the sample solution between the two temperature zones and which releases same to atmospheric pressure when fluid supply is stopped; a substrate capable of mounting a chip for a specific nucleic acid amplification; and a control mechanism for controlling the drive of each mechanism for supplying fluid when an electric signal from the fluorescence detector related to the transfer of the sample solution is transmitted. By measuring the fluorescence intensity for each thermal cycle the chip for nucleic acid amplification performs real-time PCR.SELECTED DRAWING: None 【課題】可搬型の小型で高速にリアルタイムＰＣＲが可能な、レシプロカルフロー型の核酸増幅装置の提供。【解決手段】変性温度帯と伸長・アニーリング温度帯を形成できるヒーター、前記２つの温度帯間の試料溶液の移動を検出可能な蛍光検出器、前記２つの温度帯間の試料溶液の移動を可能にし、かつ、送液停止時には大気圧開放される１対の送液用機構、特定の核酸増幅用チップを載置可能な基板、試料溶液の移動に関する蛍光検出器からの電気信号が送られて各送液用機構の駆動を制御する制御機構を備え、サーマルサイクル毎の蛍光強度の計測を行うことでリアルタイムＰＣＲを行う核酸増幅用チップ。【選択図】なし