METHOD OF SEPARATING RARE CELLS AND GENE ANALYSIS METHOD

• Main Authors: UEHARA TOSHISHIGE, NAKAMURA SEITA, ITO AKIKO, ODAGIRI TAIHEI
• Format: Patent
• Language: English; Japanese
• Published: 21.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method of separating rare cells with a high yield.SOLUTION: A method of separating rare cells comprising: a capturing step of filtering a liquid containing rare cells with a filter and capturing the rare cells on the surface of the filter; a transfer step of transferring the rare cells captured on the surface of the filter to a film-like transfer membrane which is adhesive on at least one side to hold the rare cells on one side of the transfer membrane; and a recovery step of recovering the rare cells from the transfer membrane.SELECTED DRAWING: Figure 3 【課題】希少細胞を高収率で単離する方法を提供する。【解決手段】希少細胞含有液をフィルターでろ過して希少細胞を上記フィルターの面上に捕捉する捕捉工程と、上記フィルターの面上に捕捉された上記希少細胞を、少なくとも一方の面に粘着性を有するフィルム状の転写部材に転写することで、上記希少細胞を上記転写部材の一方の面に保持する転写工程と、上記転写部材から上記希少細胞を回収する回収工程と、を含む、希少細胞の単離方法。【選択図】図３