POLYPEPTIDE, NUCLEIC ACID, PLASMID, RECOMBINANT CELL, AND METHOD OF PRODUCING ISOPRENE

• Main Authors: KAWABATA KAZUFUMI, FURUYA MASAHIRO, FUKUNISHI KANA
• Format: Patent
• Language: English; Japanese
• Published: 21.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a technology for improving the productivity of isoprene by a recombinant cell by improving the stability of isoprene synthase within a recombinant cell.SOLUTION: A polypeptide with isoprene synthase activity derived from Populus nigra comprising an amino acid sequence in which at least one amino acid present in a region A at positions 100-103, a region B at positions 261-263, or a region C at positions 464-466 is substituted for an amino acid excluding lysine and arginine is provided.SELECTED DRAWING: Figure 1 【課題】組換え細胞内におけるイソプレン合成酵素の安定性を向上させ、組換え細胞によるイソプレンの生産性を向上させる技術を提供する。【解決手段】Populus nigra由来のイソプレン合成酵素活性を有するポリペプチドであって、該ポリペプチドのアミノ酸配列における、１００〜１０３番目の領域Ａ、２６１〜２６３番目の領域Ｂ、又は４６４〜４６６番目の領域Ｃに存在する少なくとも１つのアミノ酸がリジンとアルギニンを除く他のアミノ酸に置換されたアミノ酸配列を含むポリペプチドが提供される。【選択図】図１