METHOD FOR PREPARING COMPOSITION CONTAINING ANTIBODY HIGHLY CONCENTRATED BY ULTRAFILTRATION

• Main Authors: TANAKA SAEKO, OOEDA MASAYOSHI, BENDER JEAN, WAKAYAMA RUMIKO, LAU KELBY, ISODA TOMONORI, YAMADA HIDENARI
• Format: Patent
• Language: English; Japanese
• Published: 03.08.2017
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; TRANSPORTING

PROBLEM TO BE SOLVED: To provide a method for preparing a composition containing antibodies highly concentrated by ultrafiltration.SOLUTION: A method for preparing a composition containing concentrated antibodies includes: a process (1) for preparing a supply liquid flow rate such that a value of supply liquid pressure to be applied to an ultrafiltration membrane can be raised to 85 to 100% of the designated maximum supply liquid pressure of an ultrafiltration membrane, the value of the maximum supply liquid pressure being 2.0 bar or a value exceeding 2.0 bar; and a process (2) for lowering supply liquid flow rate such that the value of the supply liquid pressure to be applied to be ultrafiltration membrane is maintained or lowered after the process (1).SELECTED DRAWING: Figure 4 【課題】限外ろ過によって高度に濃縮された抗体を含む組成物を調製する方法の提供。【解決手段】（１）限外ろ過膜に適用される供給液圧力の値が限外ろ過膜の指定された最大供給液圧力の８５〜１００％に上昇することを可能にするように、供給液流量を調節する工程であって、当該最大供給液圧力の値が２．０バールまたは２．０バールを超える値である、工程;および（２）工程（１）の後に、限外ろ過膜に適用される供給液圧力の値を維持するかまたは低下させるように、供給液流量を低下させる工程、を含む、濃縮された抗体を含む組成物を調製するための方法。【選択図】図４