METHOD OF DETECTING ANTI-VON WILLEBRAND FACTOR (VWF) ANTIBODIES

• Main Authors: KIYOKU YASUO, ICHINOSE AKITADA
• Format: Patent
• Language: English; Japanese
• Published: 13.07.2017
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method of detecting an autoantibody against the von Willebrand factor in blood, and a kit used for the detection.SOLUTION: Disclosed are a method of detecting a free autoantibody against the von Willebrand factor in a blood sample, and a kit used for the method, the method including steps of applying a sample and a labeled anti-human IgG antibody onto a support body having the von Willebrand factor solid-phased thereon to form a complex of the von Willebrand factor, an autoantibody against the von Willebrand factor, and the labeled anti-human IgG antibody on the support body, and detecting a signal from the labeled anti-human IgG antibody.SELECTED DRAWING: None 【課題】血液中のvon Willebrand因子に対する自己抗体を検出する方法及び該検出に用いるキットの提供。【解決手段】血液試料中のvon Willebrand因子に対する遊離の自己抗体を検出する方法であって、von Willebrand因子を固相化した支持体に試料及び標識した抗ヒトIgG抗体を添加し、支持体上でvon Willebrand因子-von Willebrand因子に対する自己抗体-標識した抗ヒトIgG抗体の複合体を形成させ、標識した抗ヒトIgG抗体からのシグナルを検出することを含む、方法、並びに該方法に用いるキット。【選択図】なし