FOREIGN SUBSTANCE INTRODUCTION DEVICE AND FOREIGN SUBSTANCE INTRODUCTION METHOD

• Main Authors: OGI MIEKO, AIZAWA KOJI
• Format: Patent
• Language: English; Japanese
• Published: 25.05.2017
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide foreign substance introduction technique achieving improvement in efficiency of introducing foreign substance into a cell.SOLUTION: A foreign substance introduction device 10 introduces a foreign substance into cells by applying stress waves to a cell. The foreign substance introduction device 10 comprises: cell fixing means 30 configured to temporarily fixes a cell 60 of an introduction subject onto the surface of a platy member using an electrostatic force and to release the fixation of the cell 60; and stress wave generating means 40 for irradiating the cell 60 fixed to the platy member with stress wave. The cell fixing means 30 may have a plurality of electrode portions 32 provided in a one-dimensional or two-dimensional array shape along the platy member. The stress wave generating means 40 may have a plurality of wave sources 45 provided in a one-dimensional or two-dimensional array so as to locally irradiate positions corresponding to the respective electrode portions 32 with stress waves.SELECTED DRAWING: Figure 1 【課題】細胞内への導入効率を高めた外来物質導入技術を提供する。【解決手段】外来物質導入装置１０は、応力波を作用させて細胞内に外来物質を導入する。外来物質導入装置１０は、導入対象の細胞６０を静電力を用いて板状部材の表面に一時的に固定し、その固定を解除できるように構成される細胞固定手段３０と、板状部材に固定される細胞６０に向けて応力波を照射する応力波発生手段４０と、を備える。細胞固定手段３０は、板状部材に沿って一次元または二次元アレイ状に設けられる複数の電極部３２を有してもよい。応力波発生手段４０は、各電極部３２に対応する位置に局所的に応力波を照射するように一次元または二次元アレイ状に設けられる複数の波源４５を有してもよい。【選択図】図１