LOW FUCOSE CELL LINES AND USES THEREOF

• Main Authors: DANIEL HELMAN, MIRA TOISTER-ACHITUV, MEIRAV BAR-SHIMON
• Format: Patent
• Language: English; Japanese
• Published: 18.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide methods for selecting cells with zero fucose level useful as a host cells for expressing recombinant proteins.SOLUTION: Provided is a method comprising the steps of: introducing genetic mutations into a population of Chinese hamster ovary (CHO) cells by contacting th...