PSF1 GENE EXPRESSION INHIBITOR

• Main Authors: TAKAKURA NOBUYUKI, TAJIMA TOSHIO, KOBAYASHI KOJI
• Format: Patent
• Language: English; Japanese
• Published: 02.03.2017
• Subjects: FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

PROBLEM TO BE SOLVED: To provide more effective means for treating cancer through the expression suppressive activity against the PSF1 gene.SOLUTION: The present invention provides a composition having the expression suppressive activity against the PSF1 gene. In an HPLC analysis under the conditions (A) to (F) described below, the composition comprises at least one or more components selected from the group consisting of component (I) having a peak at a retention time of 7.8 minutes, component (II) having a peak at a retention time of 9.2 minutes, component (III) having a peak at a retention time of 12.5 minutes, component (IV) having a peak at a retention time of 20.8 minutes, and component (V) having a peak at a retention time of 21.6 minutes. The components (I) to (V) are derived from cucumber or cowpea. The conditions are (A) HPLC column: a column with an inner diameter of 4.6 mm and a length of 150 mm, packed with an octadecylated silica gel carrier (a particle size of 3 μm). (B) HPLC column oven temperature: 40°C, (C) flow rate: 0.8 ml/min, (D) detection: 190 to 400 nm, (E) mobile phase: (a) methanol and (b) 2.5 mM aqueous ammonium formate solution, (F) gradient conditions: a mixed solvent of 85% (a) and 15% (b) is kept for 5 minutes, the concentration of (a) in the mixed solvent is increased from 85% to 100% over 15 minutes linearly, and then the concentration of (a) is kept at 100%.SELECTED DRAWING: None 【課題】PSF1遺伝子の発現抑制活性を通じた、より効果的ながんの治療手段を提供すること。【解決手段】下記（Ａ）〜（Ｆ）の条件でのＨＰＬＣ分析において、保持時間７．８分にピークを有する成分（I）、保持時間９.２分にピークを有する成分（II）、保持時間１２.５分にピークを有する成分（III）、保持時間２０.８分にピークを有する成分（IV）、及び保持時間２１.６分にピークを有する成分（V）から成る群より選択される１種以上の成分を含有し、前記成分（I）〜（V）がキュウリグサ又はウワバミソウに由来する、ＰＳＦ１遺伝子発現抑制活性を有する組成物：（Ａ）ＨＰＬＣカラム：オクタデシル化シリカゲル担体（粒子径３μｍ）を充填した内径４.６ｍｍ、長さ１５０ｍｍのカラム（Ｂ）ＨＰＬＣカラムオーブン温度：４０℃（Ｃ）流速：０.８ｍｌ／分（Ｄ）検出：１９０〜４００ｎｍ（Ｅ）移動相：（ａ）メタノール及び（ｂ）２.５ｍＭギ酸アンモニウム水溶液（Ｆ）グラジエント条件：８５％の（ａ）と１５％の（ｂ）との混合溶媒を５分間保持し、その後１５分かけて混合溶媒中の（ａ）の濃度を８５％から１００％に直線的に上昇させ、その後（ａ）の濃度を１００％に維持。【選択図】なし