L-GLUTAMIC ACID MEASUREMENT KIT

• Main Authors: ARAI SATOSHI, KUSAKABE HITOSHI
• Format: Patent
• Language: English; Japanese
• Published: 19.01.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To measure L-glutamic acid with high accuracy by a simple method, and to provide measurement kits using a reaction reagent of liquid state which is more stable than the solution of a lyophilized product enzyme, the kits being able to measure the concentration of L-glutamic acid, while removing the ascorbic acid, even if a sample contains ascorbic acid.SOLUTION: The present invention provides a L-glutamic acid measurement kit in a sample comprising the following (A) and (B): (A) is a reaction solution I containing ascorbate oxidase, peroxidase, and Modified Trinder's Reagent; and (B) is a reaction solution II containing L-glutamic acid oxidase, a coupler compound, and a catalase inactivator.SELECTED DRAWING: None 【課題】Ｌ−グルタミン酸を簡易な方法によって、精度よく測定すること、及び本発明の別の課題は、アスコルビン酸を含む試料であっても、アスコルビン酸を除去しつつＬ−グルタミン酸濃度を測定することができ、かつ凍結乾燥品の酵素の溶解液よりも安定な液体状の反応試薬を用いる測定キットを提供すること。【解決手段】下記（Ａ）及び（Ｂ）を含有する、試料中のＬ−グルタミン酸測定キット。（Ａ）アスコルビン酸オキシダーゼ、ペルオキシダーゼ及び新トリンダー試薬を含む反応液Ｉ、（Ｂ）Ｌ−グルタミン酸オキシダーゼ、カプラー化合物及びカタラーゼ失活剤を含む反応液ＩＩ。【選択図】なし