METHOD FOR PRODUCTION OF RECOMBINANT HUMAN IDURONATE 2-SULFATASE

• Main Authors: KAWASAKI ATSUKO, SUGIMURA ATSUSHI, MIHARA KAZUTOSHI, OTSUKI KOTA, KAMEI SHOICHIRO, HATANO YUKICHI
• Format: Patent
• Language: English; Japanese
• Published: 24.11.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide methods for highly efficiently purifying rhI2S obtained in a culture supernatant to such a high purity as permits direct use as a medical drug by using various column chromatographies.SOLUTION: The invention relates to rhI2S as a medicine which has an oligosaccharide chain linked thereto containing one or more mannose 6-phosphate residues, wherein the average number of the mannose-6-phosphate residues per rhI2S molecule is in the range of 3.7 to 5.4.SELECTED DRAWING: None 【課題】培養上清中に得られたrhI2Sを，種々のカラムクロマトグラフィーを用いて，高収率で且つ医薬としてそのまま使用できる高純度にまで精製するための方法を提供すること。【解決手段】rhI2Sであって，これに結合し１個又は２個以上のマンノース−６−リン酸残基を含んでいるオリゴ糖鎖を有するものであり，rhI2Sの１分子あたりのマンノース−６−リン酸残基の平均個数が3.7〜5.4個の範囲にある，医薬としてのrhI2S。【選択図】なし