GENE AND VARIANT ASSOCIATED WITH BM1 PHENOTYPE, MOLECULAR MARKERS, AND THEIR USE

• Main Authors: CHEN WEI, ZHENG PEIZHONG, PETER D FRIEDEMANN, SIVA P KUMPATLA, DENNIS FITZL, NATHAN J VANOPDORP, THOMAS W GREENE
• Format: Patent
• Language: English; Japanese
• Published: 15.09.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide compositions and methods of utilizing a nucleic acid molecule containing mutant CAD2 genes and/or a marker linked to mutant CAD2 genes, the compositions and methods being related to specific naturally-occurring mutant maize CAD2 genes for reducing lignin content in maize plants, and the altered genes contributing bm1 maize phenotype in a specific maize lineage.SOLUTION: The invention relates to a method for making a transgenic maize plant comprising introducing a premature stop codon into a cinnamyl alcohol dehydrogenase 2 (CAD2) gene in maize plants, wherein a premature stop codon induces and performs an AC dinucleotide insert to the exon 3 of a CAD2 gene in maize.SELECTED DRAWING: Figure 1 【課題】トウモロコシ植物におけるリグニン含量の低下の為の、特異的な天然起源の変異体トウモロコシＣＡＤ２遺伝子に関し、この変化した遺伝子は特定のトウモロコシ系統におけるｂｍ１トウモロコシ表現型に寄与する。変異体ＣＡＤ２遺伝子を含む核酸分子及び／又は変異体ＣＡＤ２遺伝子に連鎖するマーカーを利用する組成物及び方法が提供される。【解決手段】トウモロコシ植物におけるシンナミルアルコールデヒドロゲナーゼ２（ＣＡＤ２）遺伝子に未成熟終止コドンを導入するステップを含む、トランスジェニックトウモロコシ植物を作出するための方法。未成熟終止コドンが、トウモロコシにおけるＣＡＤ２遺伝子のエクソン３にＡＣジヌクレオチド挿入を誘導し行う方法。【選択図】図１