FATTY ACID RELEASE STRAIN OF CYANOBACTERIA AND PRODUCTION METHOD OF FATTY ACID-RESISTANT STRAIN

• Main Authors: KOJIMA KOJI, AICHI MAKIKO, OMATA TATSUO
• Format: Patent
• Language: English; Japanese
• Published: 05.09.2016
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENTS; ENZYMOLOGY; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS,OILS, OR FATTY ACIDS OBTAINED THEREFROM; FATTY ACIDS FROM FATS, OILS OR WAXES; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a simple method for obtaining cyanobacteria having free fatty acid high-productivity without requiring complex operations such as genetic recombination.SOLUTION: There is provided a production method of fatty acid release strains of cyanobacteria, including: a step to prepare a plurality of test sections where specimen cyanobacteria are cultured using a medium-chain or long-chain fatty acid-containing culture medium, and among which a type of the medium-chain or long-chain fatty acid contained in the culture medium differs; a step to add and culture specimen cyanobacteria to the culture medium in the plurality of the test sections; a step to select a test section where a colony is not recognized in the early stage of culture but appears when the culture is continued; and a step to collect the cyanobacteria from the colony in the selected test section.SELECTED DRAWING: None 【課題】遊離脂肪酸を高生産するラン藻を、遺伝子組換えのような煩雑な操作を要することなく、簡便な方法で得ることを課題とする。【解決手段】中鎖又は長鎖脂肪酸含有培地を用いて供試ラン藻を培養する、複数の試験区であって、培地が含有する中鎖又は長鎖脂肪酸の種類が試験区間で異なる、複数の試験区を用意するステップと、前記複数の試験区において、培地に供試ラン藻を添加し、培養するステップと、培養初期にはコロニーが認められず、培養を継続することによりコロニーが出現する試験区を選抜するステップと、選抜した試験区のコロニーからラン藻を回収するステップと、を含む、ラン藻脂肪酸放出株の作出方法が提供される。【選択図】なし