PRODUCTION METHOD OF 2-DEOXYRIBOSE-5-PHOSPHATE ESTER OR SALT THEREOF, VARIANT 2-DEOXYRIBOSE-5-PHOSPHATE ESTER SYNTHETASE, RECOMBINANT ESCHERICHIA COLI AND PRODUCTION METHOD OF 2'-DEOXYNUCLEOSIDE

• Main Authors: NAKAKUBO SHU, NATSUJI AKIKO, KAI KEIICHIRO, FUKUIRI YASUSHI, KAWANAKA TOSHIHIDE, AWANO HIROICHI, NAGAHARA KIYOTERU, TOKUDA JUNKO, ONISHI NAOTO
• Format: Patent
• Language: English; Japanese
• Published: 07.04.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a 2'-deoxynucleoside (dNS) production method suitable for industrial production.SOLUTION: The invention relates to a method for producing dNS via 2-deoxyribose (dR) by performing at least one of the following (i), (ii), and (iii): (i) subjecting divalent metal salt of 2-dehydro-3-deoxy gluconic acid (KDG) to decarboxylation response to synthesize dR, and synthesizing dNS using the obtained dR as a reaction intermediate; (ii) synthesizing dR from KDG or a salt thereof using a variant enzyme having dR synthesis ability, and synthesizing dNS using the obtained dR as a reaction intermediate; or (iii) synthesizing 2-deoxyribose-5-phosphate ester (dR5P) from dR using the variant enzyme having dR5P synthesis ability, and synthesizing dNS from the obtained dR5P.SELECTED DRAWING: None 【課題】工業生産に適した２'−デオキシヌクレオシド（ｄＮＳ）の製造方法を提供する。【解決手段】本発明は、少なくとも下記（i）、（ii）及び（iii）のいずれか１つを実行することにより、２−デオキシリボース（ｄＲ）を経由してｄＮＳを製造する方法である。（i）２−デヒドロ−３−デオキシグルコン酸（ＫＤＧ）の二価金属塩を脱カルボキシル化反応に付してｄＲを合成し、得られるｄＲを反応中間体としてｄＮＳを合成する；（ii）ｄＲ合成能を有する変異型酵素を使用して、ＫＤＧ又はその塩からｄＲを合成し、得られるｄＲを反応中間体としてｄＮＳを合成する；（iii）２−デオキシリボ−ス−５−リン酸エステル（ｄＲ５Ｐ）合成能を有する変異型酵素を使用してｄＲからｄＲ５Ｐを合成し、得られるｄＲ５ＰからｄＮＳを合成する。【選択図】なし