ANTIBODIES TO TROPONIN I AND METHODS OF USE THEREOF

PROBLEM TO BE SOLVED: To provide binding proteins binding to cardiac troponin I, antibodies to troponin I and methods of the use thereof, in particular, the use of the antibodies to detect troponin I in a patient thereby diagnosing a myocardial infarction or acute coronary syndrome.SOLUTION: In a me...

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Bibliographic Details
Main Authors TU BAILIN, ROBERT N ZIEMANN, SUSAN E BROPHY, TYNER JOAN D, HUANG DAGANG
Format Patent
LanguageEnglish
Japanese
Published 24.03.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide binding proteins binding to cardiac troponin I, antibodies to troponin I and methods of the use thereof, in particular, the use of the antibodies to detect troponin I in a patient thereby diagnosing a myocardial infarction or acute coronary syndrome.SOLUTION: In a method, a Chinese Hamster Ovary (CHO) cell line, referred to as TnI 19C7 AM1 hG1 CHO 204, designated by American Type Culture Collection (ATCC) is used to introduce a recombinant expression vector encoding both the antibody heavy chain and the antibody light chain into dhfr-CHO cells by calcium phosphate-mediated transfection, thereby constructing a CHO cell line producing antibodies to troponin I.SELECTED DRAWING: None 【課題】心臓トポロニンＩと結合する結合蛋白質、トロポニンＩに対する抗体及びこれらの使用法。特に、前抗体を使用しての患者中のトロポニンＩの検出による心筋梗塞又は急性冠症候群の診断。【解決手段】アメリカンタイプカルチャーコレクション（ＡＴＣＣ）によって指定される、ＴｎＩ１９Ｃ７ＡＭ１ｈＧ１ＣＨＯ２０４と呼ばれるチャイニーズハムスター卵巣（ＣＨＯ）細胞系を用いて、抗体重鎖と抗体軽鎖の両方をコードする組換え発現ベクターを、リン酸カルシウム仲介トランスフェクションによってｄｈｆｒ−ＣＨＯ細胞中に導入し、トロポニンＩに対する抗体を産生するＣＨＯ細胞系を構築する方法。【選択図】なし
Bibliography:Application Number: JP20150179220