FRACTIONATION LIQUID CHROMATOGRAPH DEVICE AND FRACTIONATION CONDITION SEARCH METHOD

• Main Authors: TERADA HIDETOSHI, MIWA KATSUHIKO, KUSHIBE CHIE, KATSUYAMA YUJI
• Format: Patent
• Language: English; Japanese
• Published: 22.03.2016
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PROBLEM TO BE SOLVED: To provide a fractionation liquid chromatograph device and a fractionation condition search method which facilitate setting of a fractionation condition.SOLUTION: The invention is configured so that: a sample which is subjected to component separation in a time direction in a separation column 8 is introduced to a detector 9 and a fraction collector 10; and based on a detection result of the detector 9, the respective components are fractionated and collected in the fraction collector 10. A control/processing part 20 stores chromatograph data which is obtained by executing a liquid chromatograph analysis of a fractionation condition search mode under a search condition for each kind of standard sample, chromatograph data which is obtained by executing a liquid chromatograph analysis of a fractionation mode under one or plural fractionation conditions for each kind of standard sample, and fractionation conditions of the time, by compiling the data into a database for each sample or for each component, refers to the database based on an index designated by a user and the chromatograph obtained by the liquid chromatograph analysis of the fractionation condition search mode regarding a target sample, and then determines a fractionation condition of the target sample.SELECTED DRAWING: Figure 1 【課題】分取条件の設定を容易に行うことができる分取液体クロマトグラフ装置及び分取条件探索方法を提供する。【解決手段】本発明では、分離カラム８で時間方向に成分分離させた試料を検出器９とフラクションコレクタ１０に導入し、検出器９の検出結果に基づきフラクションコレクタ１０で各成分を分画して採取する。制御・処理部２０は、各種標準試料について探索用条件の下で分取条件探索モードの液体クロマトグラフ分析を実行したときに得られたクロマトグラムデータと、各種標準試料について１ないし複数の分取条件の下で分取モードの液体クロマトグラフ分析を実行したときに得られたクロマトグラムデータ及びそのときの分取条件を、試料毎或いは成分毎にデータベース化して記憶し、ユーザが指定した指標と目的試料について分取条件探索モードの液体クロマトグラフ分析で得られたクロマトグラムとに基づきデータベースを参照して、該目的試料の分取条件を決定する。【選択図】図１