NUCLEIC ACID PURIFICATION METHOD

• Main Authors: JAN PETZEL, ROLAND FABIS, SABINE JENNRICH
• Format: Patent
• Language: English; Japanese
• Published: 24.12.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for purifying nucleic acids from a sample containing nucleic acids, and to provide kits for purifying the nucleic acids.SOLUTION: The present intimation relates to a method for purifying nucleic acids from a sample containing nucleic acids, the method comprising at least the following steps: a. bringing the sample containing nucleic acids into contact with a nucleic acid binding phase comprising protonatable groups, the protonatable groups having a pK value of 9 to 12; b. binding the nucleic acids to the nucleic acid phase at a pH (binding pH) that is at least one pH unit less than the pK value of at least one of the protonatable groups; c. eluting the nucleic acids at a pH greater than the binding pH but at least one pH unit less than the pK value of at least one of the protonatable groups. Also disclosed are corresponding kits and nucleic acid binding phases that can be used for purifying nucleic acids. 【課題】核酸を核酸含有試料から精製する方法及びキットの提供。【解決手段】核酸を核酸含有試料から精製する方法であって、少なくとも以下の工程を含む方法。ａ．核酸含有試料をプロトン化可能な基を含む核酸結合相と接触させる工程であって、プロトン化可能な基のｐＫ値が９から１２である工程、ｂ．プロトン化可能な基の少なくとも１個のｐＫ値より少なくとも１ｐＨ単位低いｐＨ（結合ｐＨ）で、核酸を核酸相に結合させる工程、ｃ．結合ｐＨを超えるが、プロトン化可能な基の少なくとも１個のｐＫ値より少なくとも１ｐＨ単位低いｐＨで、核酸を溶出させる工程。核酸の精製に使用することができる対応するキット及び核酸結合相。【選択図】なし