CELL CULTURE APPARATUS

• Main Author: WADA YOSHINORI
• Format: Patent
• Language: English; Japanese
• Published: 07.12.2015
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a cell culture apparatus capable of preventing cells from adhering to a column outer surface of a portion above the fluid surface of a culture fluid during cell cultivation.SOLUTION: A cell culture apparatus includes: a cylindrical culture tank; a column standing upright from the center of the inner surface of a bottom part of the culture tank; and stirring means having a plate-like vertically installed stirring blade whose upper part is fixed to an attachment part rotatably attached to an upper portion of the column. The column has a conical portion formed into a conical shape having a rising portion from the inner surface of the bottom part, a diameter of the rising portion expanding in a direction toward the inner surface of the bottom part, and the upper portion of the column is provided above the fluid surface of a culture fluid. The stirring blade in the stirring means is formed in a shape in which a portion of the stirring blade facing the column is provided along the outer surface of the column. The stirring blade forms a widening part which prevents the culture fluid from entering a portion almost above the fluid surface of the culture fluid, which is a gap between a portion of the stirring blade facing the column and the outer surface of the column, through a capillary phenomenon when cultivating cells. 【課題】 細胞培養に際し、培養液の液面の上方の部分の支柱外面に細胞が接着するのを防止し得る細胞培養装置を提供すること。【解決手段】 円筒形状の培養槽と、培養槽の底部の内面の中央から直立する支柱と、支柱の上部分に回転可能に取り付けられる取付部にその上部が固着された板状で垂設の撹拌翼とを有する撹拌手段とを備え、支柱は底部の内面からの立ち上がり部分が底部の内面に向かう方向に拡径の円錐状に形成された円錐状部分を有し、支柱の上部分は培養液の液面より上にあり、撹拌手段の撹拌翼は撹拌翼の支柱に対向する部分が支柱の外面に沿うような形状に形成されている、細胞培養装置において、撹拌翼は、撹拌翼の支柱に対向する部分と支柱の外面との間の間隙の、培養液の略液面より上方の部分に、細胞培養時に培養液が毛細管現象により入り込むのを妨げる拡幅部が形成されている、細胞培養装置である。【選択図】図１