GLUCOSIDASE I VARIANTS WITH IMPROVED PROPERTIES

• Main Authors: THIJS KAPER, JOHAN VAN LIESHOUT, RONALDUS WILHELMUS HOMMES, PIM VAN DE KLEY, BOTT RICHARD R, KELEMEN BRADLEY, SLAVKO KRALJ, MATS SANDGREN, IGOR NIKOLAEV, FRITS GOEDEGEBUUR, SANDER VAN STIGT-THANS, PAULIEN KRUITHOF, GUDRUN VOGTENTANZ
• Format: Patent
• Language: English; Japanese
• Published: 26.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide -glucosidase 1 (BGL1) variants with improved properties.SOLUTION: The BGL1 variants of the invention have at least two improved activities over wild type BGL1 selected from: (a) hydrolytic activity on pretreated corn stover (PCS); (b) cellobiase activity; (c) protein expression; (d) BGL activity measured by either cellobiase activity in the presence of corncob (CC) pretreated with ammonia or CC hydrolytic activity; (e) thermostability; (f) hydrolytic activity on phosphoric acid swollen cellulose (PASC) and (g) hydrolytic activity in the presence of glucose. 【課題】改良された −グルコシダーゼ（ＢＧＬ１）の提供。【解決手段】ＢＧＬ１変異体であって、（ａ）前処理済みトウモロコシ茎葉（ＰＣＳ）加水分解活性、（ｂ）セロビアーゼ活性、（ｃ）タンパク質発現、（ｄ）アンモニアで前処理したトウモロコシ穂軸（ＣＣ）の存在下でのセロビアーゼ活性又はＣＣ加水分解活性のいずれかにより測定されるＢＧＬ活性、（ｅ）熱安定性、（ｆ）リン酸膨潤セルロース（ＰＡＳＣ）加水分解活性、及び（ｇ）グルコース存在下での加水分解活性、から選択される少なくとも２つの活性が野生型ＢＧＬ１よりも改良されているＢＧＬ１変異体。【選択図】なし