DEVICE AND METHOD FOR CONTROLLING THE AMOUNT OF REAGENT TO BE INJECTED IN MICROCHIP

• Main Authors: UEKI TATSUYA, KUROKI HIROYUKI
• Format: Patent
• Language: English; Japanese
• Published: 02.11.2015
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PROBLEM TO BE SOLVED: To provide a technique to inject a reagent in a microchip passage each time at a constant amount.SOLUTION: The device and method for performing biochemical analysis comprise an installation part on which a microchip is installed, a nozzle part for injecting a reagent in an inlet of the microchip, a reagent discharge part for discharging or sucking the reagent, a detection part for detecting the end edge of an injected liquid in the microchip, and a control part for controlling the discharge and sucking amount of the reagent discharge part on the basis of information obtained by the detection part. When the reagent is injected in the microchip, if a position of the end edge of the injected liquid in a microchip main passage, which is detected by the detection part, exceeds a proper position, the injected liquid is sucked by decompressing the reagent discharge part to return the position of the end edge of the injected liquid to the proper position, and if the position of the end edge of the injected liquid in the microchip passage does not reach the proper position, the injected liquid is increased by additionally compressing the reagent discharge part to control the position of the end edge of the injected liquid to reach to the proper position. 【課題】マイクロチップ流路内への試薬注入量を毎回一定量にする技術を提供する。【解決手段】マイクロチップを設置する設置部と、前記マイクロチップ注入口へ試薬を注入するノズル部と、試薬を吐出・吸引させる試薬吐出部と、前記マイクロチップの注入液終端を検出する検出部と、前記検出部で得た情報を元に試薬吐出部に対して吐出・吸引量を制御する制御部を備え、前記マイクロチップへ試薬を注入した際、前記検出部にて検出したマイクロチップ主流路内の注入液終端位置が、適正位置より行き過ぎている場合は、前記試薬吐出部を減圧させて注入液を吸引し、注入液終端位置を適正位置まで戻し、逆に前記マイクロチップ流路内の注入液終端位置が適正位置に達していない場合は、前記試薬吐出部を追圧させて注入液を増加させ、注入液終端位置を適正位置に達するように制御する生化学分析装置及び方法を提供する。【選択図】図１