AMPHIBIAN XENOPUS TROPICALIS SKIN-DERIVED FUNCTIONAL PEPTIDES

• Main Authors: HAGIWARA YOSHIHISA, IMURA TOMOHIRO, HARAMOTO ETSUKAZU, WATABE KUNIHIKO, SHIGERI YASUSHI, ITO YUZURU, ASAJIMA MAKOTO
• Format: Patent
• Language: English; Japanese
• Published: 05.10.2015
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

PROBLEM TO BE SOLVED: To provide new functional peptides that are expected to be useful for the biophylaxis of frogs such as antibacterial action or the like, the new functional peptides being derived from frog skin, specifically amphibian Xenopus tropicalis skin.SOLUTION: This invention provides any of peptides named Pxt-1 to Pxt-11, respectively. Amino-acid sequences of the Pxt-1 to the Pxt-11 are as follows. Pxt-1: IRPIPFIPRGGKT, Pxt-2: FIGALLRPALKLLA, Pxt-3: GLKEVAHSAKKFAKGFISGLTGS, Pxt-4: LKGASKLIPHLLPSRQQ, Pxt-5: FIGALLGPLLNLLK, Pxt-6: IRPVPFFPPVHAKKVFPLH, Pxt-7: Pyr-GLIGTLTAKQIKK, Pxt-8: IRPIPFIPR, Pxt-9: GLKEVAHSAKKF, Pxt-10: LMGTLISKQMKK, and Pxt-11: Pyr-GLMGTLISKQMKK. Here, Pyr is a pyroglutamic acid residue, and C terminals of the Pxt-1 to the Pxt-11 are each independently COOH or CONH. 【課題】カエルの皮膚、詳しくは両生類ネッタイツメガエル皮膚由来の抗菌作用などのカエルの生体防御に有用な新規な機能性ペプチドの提供。【解決手段】以下のいずれかのペプチド。Pxt-1:IRPIPFIPRGGKT、Pxt-2:FIGALLRPALKLLA、Pxt-3:GLKEVAHSAKKFAKGFISGLTGS、Pxt-4:LKGASKLIPHLLPSRQQ、Pxt-5:FIGALLGPLLNLLK、Pxt-6:IRPVPFFPPVHAKKVFPLH、Pxt-7:Pyr-GLIGTLTAKQIKK、Pxt-8:IRPIPFIPR、Pxt-9:GLKEVAHSAKKF、Pxt-10:LMGTLISKQMKK、Pxt-11:Pyr-GLMGTLISKQMKK（ここで、Pyrはピログルタミン酸残基を示し、C末端はOHまたはNH2である）【選択図】図２