METHOD FOR MANUFACTURING PLURIPOTENT STEM CELL WITH IMPROVED CHIMERA FORMING ABILITY FROM PRIME-TYPE PLURIPOTENT STEM CELL, AND COMPOSITION AND COMBINATION FOR THE SAME

• Main Authors: NAKAUCHI HIROMITSU, MURAYAMA HIDEYUKI, MASAKI HIDEKI
• Format: Patent
• Language: English; Japanese
• Published: 30.07.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for bringing a prime-type pluripotent stem cell to a more undifferentiated state, a method for manufacturing a pluripotent stem cell with improved chimera forming ability from a prime-type pluripotent stem cell, and a method for manufacturing a chimeric animal by using a pluripotent stem cell obtained by the method.SOLUTION: A method includes bringing a prime-type pluripotent stem cell to a Wnt/ -catenin signal suppressed state and a signal transducer and activator of transcription 3 (STAT3) signal activated state. Further, a method includes introducing the pluripotent stem cell manufactured by the method into an embryo of mammal. 【課題】プライム型多能性幹細胞をより未分化な状態にする方法、プライム型多能性幹細胞からキメラ形成能を向上させた多能性幹細胞を製造する方法、および、これらの方法により得られた多能性幹細胞を用いてキメラ動物を作製する方法を提供する。【解決手段】プライム型多能性幹細胞をＷｎｔ／ カテニンシグナル抑制状態、かつシグナル伝達及び転写活性化因子３（ＳＴＡＴ３）シグナル活性化状態にすることを含んでなる、方法。また、前記方法により製造された多能性幹細胞を哺乳動物の胚に導入することを含んでなる、方法。【選択図】なし