HOST CELLS AND METHODS OF USE THEREOF

• Main Authors: ZHU YUAN, JAMES D JOWETT, JIN YONG HWAN, ALEXANDER H TAYLOR
• Format: Patent
• Language: English; Japanese
• Published: 27.07.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide genetically modified Pichia strains in which polypeptides as target product material do not face barriers of undesired proteolysis and glycosylation; and methods of producing the polypeptides.SOLUTION: In the genetically modified Pichia strains, nucleic acid sequences PEP4, PRB1, YPS1, YPS2, YMP1, YMP2, YMP3 and PMT4 are genetically modified which are responsible for proteolysis and/or glycosylation in the strains and, in particular, encode a functional gene product or expression of the gene product. 【課題】目的の生産物質であるポリペプチドが好ましくないタンパク質分解および糖鎖形成による障害を受けない遺伝子改変ピキア株及びポリペプチドを産生する方法の提供。【解決手段】遺伝的修飾ピキア株においてタンパク質分解及び／又は糖鎖形成を担う、特に、機能的遺伝子産物又は前記遺伝子産物の発現をコードする核酸配列であるＰＥＰ４、ＰＲＢ１、ＹＰＳ１、ＹＰＳ２、ＹＭＰ１、ＹＭＰ２、ＹＭＰ３及びＰＭＴ４が遺伝的に修飾されるピキア株。【選択図】なし