NUCLEIC ACID MOLECULE BOUND TO RACTOPAMINE AND APPLICATION THEREOF

• Main Authors: WAGA IWAO, MINAGAWA HIROTAKA, YOSHIDA YOSHIHITO, AKITOMI MINORU, HORII KATSUNORI, SHIRATORI YUKIHIRO, FURUICHI MAKIO
• Format: Patent
• Language: English; Japanese
• Published: 09.04.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a nucleic acid molecule available for detection of ractopamine, which is an agonist to adrenoreceptor which is not approved in Japan, in meat or internal organs of cattle.SOLUTION: The invention provides a nucleic acid molecule comprising at least one polynucleotide selected from the group consisting of following (a)-(c), and the polynucleotide binds the nucleic acid containing at least one modified base to ractopamine. (a) is a polynucleotide consisting of a specific base sequence. (b) is a polynucleotide bound to ractopamine consisting of a base sequence at least 80% identical to the above-mentioned base sequence. (c) is a polynucleotide bound to ractopamine consisting of a base sequence complementary to a polynucleotide which hybridizes under stringent conditions to the polynucleotide consisting of the base sequence of the (a). 【課題】国内で承認されていない アドレナリン受容体に対する作動薬のラクトパミンの家畜の肉や内臓における検出に利用可能な核酸分子を提供する。【解決手段】下記（ａ）〜（ｃ）からなる群から選択された少なくとも一つのポリヌクレオチドを含み、前記ポリヌクレオチドが、少なくとも１つの修飾塩基を含む核酸分子を、ラクトパミンに結合する核酸分子とする。（ａ）特定の塩基配列からなるポリヌクレオチド（ｂ）前記の塩基配列に対して、８０％以上の同一性を有する塩基配列からなり、ラクトパミンに結合するポリヌクレオチド（ｃ）前記（ａ）の塩基配列からなるポリヌクレオチドに対してストリンジェントな条件下でハイブリダイズするポリヌクレオチドに相補的な塩基配列からなり、ラクトパミンに結合するポリヌクレオチド【選択図】図１