METHOD OF NORMALIZED QUANTIFICATION OF NUCLEIC ACIDS USING ANCHOR OLIGONUCLEOTIDES AND ADAPTER OLIGONUCLEOTIDES

• Main Authors: LOEFFERT DIRK, KORAGE CHRISTIAN, ENGEL HOLGER
• Format: Patent
• Language: English; Japanese
• Published: 26.02.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides.SOLUTION: The present invention relates to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to a method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample. The invention also relates to a kit for a normalized quantification of nucleic acids in a sample, the kit comprising: one or more first nucleic acid probes substantially complementary to defined regions of nucleic acid or a specific class of nucleic acids in the sample, wherein the first nucleic acid probe comprises one or more primer binding sites and a probe binding site; and a second nucleic acid probe substantially complementary to the probe binding site on the first nucleic acid probe. 【課題】アンカーオリゴヌクレオチドおよびアダプターオリゴヌクレオチドを用いる核酸の正規化した定量化方法の提供。【解決手段】本発明は、核酸の正規化された定量化、および試料、例えば、核酸の混合物における核酸の数量についての正規化に関する。本発明は、試料中の定量化される核酸の数量を、試料中の核酸の総数量、または試料中の特定のクラスの核酸の総数量に対して正規化するための方法に関する。本発明は、試料中の核酸の正規化された定量化のためのキットであって、試料中の核酸の限定された領域または特定のクラスの核酸と実質的に相補的な１種以上の第１の核酸プローブであって、１種以上のプライマー結合部位および１つのプローブ結合部位を含む第１の核酸プローブ；ならびに前記第１の核酸プローブの前記プローブ結合部位と実質的に相補的な第２の核酸プローブを含むキットにも関する。【選択図】図１