SELF-ASSEMBLING MULTICELLULAR BODIES AND METHODS FOR PREPARING THREE-DIMENSIONAL BIOLOGICAL STRUCTURES USING THE SAME

• Main Authors: CYRILLE NOROTTE, GABOR FORGACS, FRANCOISE SUZANNE MARGA
• Format: Patent
• Language: English; Japanese
• Published: 05.02.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide means enabling construction of three dimensional vascularized organs.SOLUTION: Structures and methods for tissue engineering include a multicellular body including a plurality of living cells. A plurality of multicellular bodies can be arranged in a pattern and allowed to fuse to form an artificial tissue. The arrangement can include filler bodies including a biocompatible material that resists migration and ingrowth of cells from the multicellular bodies and also resists adherence of cells to it. Three-dimensional constructs can be assembled by printing or otherwise stacking the multicellular bodies and filler bodies such that there is direct contact between adjoining multicellular bodies, suitably along a contact area of a substantial length. The direct contact between the multicellular bodies promotes efficient and reliable fusion. The increased contact area between adjoining multicellular bodies also promotes efficient and reliable fusion. 【課題】３次元の血管新生化臓器を組み立てることを可能にする手段を提供する。【解決手段】ティッシュエンジニアリングのための構造体と方法は、複数の生体細胞を含む多細胞体を含む。複数の多細胞体をあるパターンで配列して融合させて、人工組織を形成させることができる。配列体は、細胞が多細胞体から移動および内殖するのを妨げると共に、細胞が多細胞体に接着するのを妨げる生体適合性物質を含むフィラー体を含むことができる。好適には十分な長さのある接触区域沿いで、隣接し合う多細胞体が直接接触するように、多細胞体とフィラー体を印刷するか、または他の方法によって積み重ねることによって、３次元コンストラクトを組み立てることができる。このように多細胞体間を直接接触させることによって、効率的かつ確実な融合を促す。また、隣接し合う多細胞体間の接触面積を増大させることによっても、効率的かつ確実な融合を促す。【選択図】図９