CELL SEPARATION METHOD AND CELL SEPARATION DEVICE

• Main Author: KOMURA YUKIO
• Format: Patent
• Language: English; Japanese
• Published: 15.12.2014
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS; SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS; SPIRITS; TESTING; TRANSPORTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a cell separation method and the like which enables cells to be easily separated from cell solution containing aggregated cells using the same device.SOLUTION: A valve 19a is opened, while a valves 19b, 19c are closed. After a solution holding part 5 is filled with a predetermined amount of cell-containing solution, the cell-containing solution is sent to a cell separation part 7a when pressure is given to the cell-containing solution in the solution holding part 5. The cell-containing solution having sent to the cell separation part 7a is sent to a cell separation part 7b, passing through a filter 23 arranged in the cell separation part 7a. For example, cell-containing solution after separating and collecting a cell having size of 100 μm or more (aggregated cell) is retained in a solution retention part 9. When a predetermined amount of the cell-containing solution is retained in the solution retention part 9, the valve 19a is closed and the valves 19b, 19c are released. Then the cell-containing solution in the solution retention part 9 is sent out to a flow passage 21 that is connected to a cell separation part 15 by adding pressure from a releasing part 11. 【課題】 塊状細胞を含む細胞溶液から、同一の装置によって、細胞を容易に分離することが可能な細胞分離方法等を提供する。【解決手段】 弁１９ａを開き、弁１９ｂ、１９ｃは閉じておく。所定量の細胞含有溶液を溶液保持部５に充填した後、溶液保持部５内の細胞含有溶液に圧力を付与すると、細胞含有溶液が細胞分離部７ａに送られる。細胞分離部７ａに送られた細胞含有溶液は、細胞分離部７ａ内に配置されたフィルター２３を通過して、細胞分離部７ｂへ送られる。例えば１００μｍ以上のサイズの細胞（塊状細胞）を分離回収した後の細胞含有溶液は、溶液貯留部９に貯留される。所定量の細胞含有溶液が溶液貯留部９に貯留すると、次に、弁１９ａを閉じ、弁１９ｂ、１９ｃを解放する。次に、解放部１１から圧力を加えることで、溶液貯留部９内の細胞含有溶液が細胞分離部１５へつながる流路２１へ送り出される。【選択図】図１