POLYPEPTIDE

• Main Authors: FIORESI CAROL, GERRITSE GIJSBERT, LIU WEI, SOERENSEN BO SPANGE, KRAGH KARSTEN MATHIAS, KELLET-SMITH ANJA HEMMINGEN, THOUDAHL CHARLOTTE REFDAHL, DERKX PATRICK M F, BERG CASPER TUNE, SHAW ANDREW
• Format: Patent
• Language: English; Japanese
• Published: 27.11.2014
• Subjects: BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; EDIBLE DOUGHS; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PRESERVATION THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a polypeptide (particularly, amylase polypeptide) that prevents hardening of bread due to the crystallization of amylopectin generated in starch granules with time, also to provide nucleic acid encoding them, and its use as non-maltogenic exoamylase in food manufacturing.SOLUTION: Disclosed are PS4 variant polypeptides derivable from a parental polypeptide having non-maltogenic exoamylase activity, which have amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70 145, 188, 272 and 339 with reference to the position numbering of a specified sequence of Pseudomonas saccharophilia exoamylase. 【課題】経時変化に伴い澱粉の顆粒中に生じるアミロペプチンの結晶化に伴う、パンの硬化を防止する、ポリペプチド（詳細にはアミラーゼポリペプチド）及びこれらをコードする核酸、並びに食品を製造する際の非マルトース生成エキソアミラーゼとしてのそれらの使用を提供。【解決手段】非マルトース生成エキソアミラーゼ活性を有する親ポリペプチドから誘導可能なＰＳ４改変体ポリペプチドであって、Ｐｓｅｕｄｏｍｏｎａｓ ｓａｃｃｈａｒｏｐｈｉｌｉａエキソアミラーゼの特定の配列の位置番号、１２１、１６１、２２３、１４６、１５７、１５８、１９８、２２９、３０３、３０９、３１６、３５３、２６、７０、１４５、１８８、２７２、３３９からなる群から選択される１つ以上の位置でのアミノ酸突然変異を含むＰＳ４改変体ポリペプチド。【選択図】なし