COMPOSITION FOR PRODUCING INDUCED PLURIPOTENT STEM CELL, AND METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELL

• Main Authors: ISEKI HIROTAKA, OKUDA AKIHIKO, OKAZAKI YASUSHI
• Format: Patent
• Language: English; Japanese
• Published: 20.11.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for producing induced pluripotent stem cells which is excellent in production efficiency, which can be produced in a short period of time, and which is also excellent in quality aspects, and to provide a composition for producing induced pluripotent stem cells.SOLUTION: A method for producing induced pluripotent stem cells comprises the steps of introducing Oct3/4 genes or a gene product thereof, Sox2 genes or a gene product thereof, Klf4 genes or a gene product thereof, c-Myc genes or a gene product thereof, and Jarid2 mutant genes or a gene product thereof into somatic cells. The Jarid2 mutant gene or a gene product thereof is a gene encoding amino acids from the first to the 551st of the N terminal of a Jarid2 protein or a gene product thereof. A composition for producing induced pluripotent stem cells comprises the Jarid2 mutant gene encoding amino acids from the first of the N terminal of Jarid2 protein to the 551st or a gene product thereof. 【課題】製造効率に優れ、短期間で製造することができ、品質面にも優れた人工多能性幹細胞の製造方法、及び人工多能性幹細胞製造用組成物の提供。【解決手段】Ｏｃｔ３／４遺伝子乃至その遺伝子産物、Ｓｏｘ２遺伝子乃至その遺伝子産物、Ｋｌｆ４遺伝子乃至その遺伝子産物、ｃ−Ｍｙｃ遺伝子乃至その遺伝子産物、及びＪａｒｉｄ２変異体遺伝子乃至その遺伝子産物を体細胞に導入する工程を含み、前記Ｊａｒｉｄ２変異体遺伝子乃至その遺伝子産物が、Ｊａｒｉｄ２タンパク質のＮ末端の１番目から５５１番目のアミノ酸をコードする遺伝子乃至その遺伝子産物である人工多能性幹細胞の製造方法、Ｊａｒｉｄ２タンパク質のＮ末端の１番目から５５１番目のアミノ酸をコードするＪａｒｉｄ２変異体遺伝子乃至その遺伝子産物を含む人工多能性幹細胞製造用組成物である。【選択図】なし